Part:BBa_K2500012:Design
AND Gate C: Synthetic Promoter Responsive to LldR and LuxR
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We reasoned that flanking the pLux promoter with O1 and O2 should result in an AND-gate behavior and relied on the previously characterized parts BBa_K1847007 (O1-pconst-O2), BBa_R0062 (plux) and BBa_C0062 (LuxR).
In design C of the AND gate, we adapted the architecture of BBa_K1847007. The Anderson promoter was exchanged with the pLux promoter such that a) the -35 and -10 regions of the new promoter were at the same position as that of the original one and that b) the total distance between O1 and O2 was kept the same as in the original part. Further, we inserted a synthetic DNA spacer between the pLux promoter and the O2 LldR binding site to increase the flexibility of the looping region in case the replacement of the original Anderson promoter of BBa_K1847007 with pLux hampers the looping process.
AND gate C was ordered as a gBlock with flanking restriction sites, allowing it to be combined with our downstream elements via restriction-ligation cloning.
Source
This part was synthetically designed and ordered as a g-block. This part was designed based on the previously characterized parts BBa_K1847007 (LldP/LldR, Plldr) and BBa_R0062 (Plux)/ BBa_C0062 (LuxR).